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Goat anti-Chicken IgG , CY3 AffiniPure
貨號:PMK-015-097M/PMK-015-097S
一鍵復制產品信息
價格:
240.00
規格:
100ul
1ml
貨期:
現貨
本產品4℃運輸,保存于-20℃,保質期 12個月。
Details
產品詳情
  • Applications

    IF

  • Species Cross-Reactivity

    Chicken

  • Molecular Weight

    150KD

  • Isotype

    IgG1

  • Storage Buffer & Condition

    0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6 Store at -20°C. 

    Do not aliquot the antibody.


  • Stabilizer

    2mg/ml

  • Specificity

    Goat Polyclonal IgG, affinity purified by Protein A.

  • Suggested Dilution Range

    IF=1:100-1000

  • Fluorophore

    Cy3 bis-NHS ester Amax=514nm,552 nm; Emax=570 nm

  • Purity

    The antibody was purified from antisera by immunoaffinity chromatographyusing antigens coupled to 

    agarose beads.


  • Antibody Specificity

    Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 

    mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are 

    produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two 

    separate functions; to bind to the pathogen that elicited the response and to recruit other cells and 

    molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination 

    and the number of specificities in an individual at a given time point is estimated to be 1011 variants.


  • Expiration date

    Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results 

    are acceptable for the intended use.


  • Note

    For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.

Instruction manual/COA download
說明書/COA下載
Published literature
已發表文獻
  • 更新中...

Product Q&A FAQ
產品問答FAQ
  • Western Blot(WB)實驗中有很多雜帶咋回事?

    答:可能的原因有:

    1、目的蛋白有多個修飾位點,本身可以呈現多條帶,建議查閱文獻或進行生物信息學分析,獲得蛋白序列的修飾位點信息,通過去修飾確定蛋白實際大小;

    2、樣本處理過程中目的蛋白發生降解,建議加入蛋白酶抑制劑;樣本處理時在冰上操作;

    3、雜蛋白多,建議處理目的蛋白;

    4、抗體特異性不強,建議使用特異性強的抗體;

    5、抗體孵育時間過久,建議減少抗體孵育時間;

    6、二抗與抗原有交叉反應,建議選擇合適的封閉物;

    7、二聚體或多聚體存在,建議增加蛋白質變性過程及強度;

    8、底物顯色與曝光時間過長,建議縮短顯色及曝光的時間。


Copyright ?湖北普美生物科技有限公司 All Rights Reserved.    工信部備案:鄂ICP備17001029號-1    技術支持:武漢微盟

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