
Storage Buffer : 0.1M Sodium phosphate, 0.15M NaCl, 50% glycerol, pH7.4
Stabilizer: 1% Bovine Serum Albumin (IgG-Free,Protease-Free)
Preservative: 0.02% Sodium Azide
Suggested Dilution Range: 1:100-1:800 for most applications
Optimal dilutions should be determined by the end user.
Fluorophore: Dylight 680--TFP ester
Amax=682nm; Emax=715nm
Purity:The antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins.
Antibody Source:The antibody was affinity purified from antisera by immunoaffinity chromatography using purified mouse IgG.
Expiration date:Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are acceptable for the intended use.
更新中...
答:可能的原因有:
1、細(xì)胞中不表達(dá)這種蛋白質(zhì),換一種細(xì)胞;
2、抗體不能識(shí)別目標(biāo)蛋白,多看看說(shuō)明,是否有問(wèn)題;
3、可能是沒(méi)有保持低溫操作,樣品保存不當(dāng),樣品放置時(shí)間過(guò)長(zhǎng);
4、細(xì)胞中的蛋白質(zhì)被酶降解掉了,可加入蛋白酶抑制劑,抑制蛋白酶活性。
答:一般5×10^6就足夠。
1、可以加大抗原上樣量,這是主要的;
2、也可以將一抗稀釋比例降低;
3、還可以延長(zhǎng)曝光時(shí)間。
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