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WB, IP, IF

N/A

N/A

IgG1

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. 

Do not aliquot the antibody.

WB: 1:5,000   

IP: 1:200      

IF: 1:1000

The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins.

The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity 

or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. 

It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein 

expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can 

be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, 

adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.