WB, IHC
H, R, M, Mk, Ch, Hm, Sh，Pg，Xenopus
37KD
IgG1
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.Store at -20°C.
Do not aliquot the antibody.
WB: 1:5,000
IHC: 1:200
Antibody can detects endogenous GAPDH protein.
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis.
GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as
loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH
expression in certain cell types.
1.Xiao-Long W ,Shuo W ,Zhi-Zhong W , et al.Overexpression of ATAD2 indicates Poor Prognosis in Oral Squamous Cell Carcinoma.[J].International journal of medical sciences,2020,17(11):1598-1609.(IF 2.523)
2.Bing H ,Yi Y ,Lei T , et al.Roles of SET7/9 and LSD1 in the Pathogenesis of Arsenic-induced Hepatocyte Apoptosis.[J].Journal of clinical and translational hepatology,2021,9(3):364-372.(IF 4.108)
3.Liyuan H ,Fangfang S ,Hualing S , et al.IRX5 promotes NF-κB signalling to increase proliferation, migration and invasion via OPN in tongue squamous cell carcinoma.[J].Journal of cellular and molecular medicine,2018,22(8):3899-3910.(IF 4.302)
4.Xueqing Z ,Wushuang H ,Zhenru H , et al.Effects of Fam83h truncation mutation on enamel developmental defects in male C57/BL6J mice.[J].Bone,2022,166116595-116595.(IF 4.626)
5.De-Run C ,Yuan G ,Yao X , et al.Engineered nanogels simultaneously implement HDAC inhibition and chemotherapy to boost antitumor immunity via pyroptosis[J].Applied Materials Today,2022,26(IF 8.663)
6.He F ,Wang F ,Xiang H , et al.Activation of adenosine A2B receptor alleviates myocardial ischemia-reperfusion injury by inhibiting endoplasmic reticulum stress and restoring autophagy flux.[J].Archives of biochemistry and biophysics,2024,754109945-109945.(IF 3.8)
7. Jinmeng Sun, Minmin Sun, Zekun Li, GelMA@ginsenoside Rb3 Targets Inflammatory Microenvironment in Periodontitis via MAPK Pathway. (IF 5.3)
答：可能的原因有：
1、目的蛋白有多個修飾位點，本身可以呈現多條帶，建議查閱文獻或進行生物信息學分析，獲得蛋白序列的修飾位點信息，通過去修飾確定蛋白實際大小；
2、樣本處理過程中目的蛋白發生降解,建議加入蛋白酶抑制劑；樣本處理時在冰上操作；
3、雜蛋白多，建議處理目的蛋白；
4、抗體特異性不強，建議使用特異性強的抗體；
5、抗體孵育時間過久，建議減少抗體孵育時間；
6、二抗與抗原有交叉反應，建議選擇合適的封閉物；
7、二聚體或多聚體存在，建議增加蛋白質變性過程及強度；
8、底物顯色與曝光時間過長，建議縮短顯色及曝光的時間。
Copyright ?湖北普美生物科技有限公司 All Rights Reserved. 工信部備案：鄂ICP備17001029號-1 技術支持：武漢微盟
