
PMK562M PMK562S

50ul 100ul

WB, IHC

Recombinant Strep Tag II Specific

Strep Tag II

IgG1

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.Store at -20°C.
Do not aliquot the antibody.

WB: 1:5,000 IHC: 1:200

The Strep Tag II Mouse Monoclonal Antibody can detects Strep Tag II recombinant protein.

Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide used in numerous molecular biological
protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep tag II) is a nine amino acid peptide with high specificity
and affinity towards streptavidin. NWSHPQFEK Tag Antibody is a useful tool in analysis and affinity purification of Strep tag
II fusion proteins.

Flag-Tag Mouse Monoclonal Antibody(2C5)
(PMK001M /PMK001S)
HA Mouse Monoclonal Antibody(1B10)-HRP conjugated
(PMK061M /PMK061S)
His-Tag Rabbit Polyclonal Antibody
(PMK111M /PMK111S)
C-Myc-Tag Mouse Monoclonal Antibody(3E8)
(PMK012M /PMK012S)
GAPDH Mouse Monoclonal Antibody(2B8)-HRP conjugated
(PMK053M /PMK053S)
Lamin B1 Mouse Monoclonal Antibody(7C11)
(PMK049C /PMK049F)
β-actin Mouse Monoclonal Antibody(5B7)-HRP conjugated
(PMK058M /PMK058S)
Rabbit anti-Goat IgG (H+L), HRP Conjugated
(PMK-014-097M /PMK-014-097S)

WB-Western blot
IHC-Immunochemistry
IF-Immunofluorescence
IP-Immunoprecipitation
ChIP-Chormatin Immunoprecipitation

H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog
Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
更新中...
答:可能的原因有:
1、目的蛋白有多個修飾位點,本身可以呈現多條帶,建議查閱文獻或進行生物信息學分析,獲得蛋白序列的修飾位點信息,通過去修飾確定蛋白實際大小;
2、樣本處理過程中目的蛋白發生降解,建議加入蛋白酶抑制劑;樣本處理時在冰上操作;
3、雜蛋白多,建議處理目的蛋白;
4、抗體特異性不強,建議使用特異性強的抗體;
5、抗體孵育時間過久,建議減少抗體孵育時間;
6、二抗與抗原有交叉反應,建議選擇合適的封閉物;
7、二聚體或多聚體存在,建議增加蛋白質變性過程及強度;
8、底物顯色與曝光時間過長,建議縮短顯色及曝光的時間。
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